Hundreds of Upper West Side residents and business owners are fighting back against the city’s plan to build a new bike lane along a major local thoroughfare.

The “No 72nd Street Bike Lane Coalition” has gathered more than 1,000 signatures on a petition opposing the proposal, and said it expects at least 100 people to picket against it at a rally Saturday morning at West 72nd Street and Broadway.

“This is about making sure the community is heard,” the group said in a statement. “We are calling for a pause. Release the data, evaluate the alternatives and work with the people who know this street best — the neighbors.”

DOT wants to put a bidirectional bike lane on West 72nd Street.…