Hi everyone, just want to preface by saying i am very much a microbiologist and know extremely little in the world of stats. I’ll give some context to my experiment: I preformed an enrichment where i grew bacteria in vials (n= 3) for 6 weeks, then took a portion of those growths and added it into new vials (n=3) for another 6 weeks. The vials let’s say A1, B1, C1 corresponded to the second batch of vials A2, B2, C2 where in the samples from A1 was added to A2 etc. I did this at two different temperatures (20 and 30 degrees). So i have two variables, the time and the temperature, giving me four groups.

Group 1. 20 degrees celsius week 6 (rep1, rep2, rep3)

Group 2. 30 degrees celsius week 6 (rep1, rep2, rep3)

Group 3. 20 degrees celsius week 12 (rep1, rep2, rep3)

Group 4.…