With billions of people experiencing hunger in 2025 and unable to afford a healthy diet, food insecurity remains a critical global challenge. “µBites", originally developed for the NASA Deep Space Food Challenge, represents a novel way of producing nutritious food by converting plastic and plant waste into edible, protein-rich supplements. Beyond space travel, µBites could offer a promising solution to address both food shortage and plastic pollution simultaneously. We have previously demonstrated the successful 3D printing of edible, µBites protein cookies using plastic-derived substrates and yeast biomass. In this study, we demonstrate the enhancement of flavor, aroma, and color of these cookies using naturally produced ingredients by safe-to-eat yeast strains, Saccharomyces boulardii, S. cerevisiae, and Rhodosporidium toruloides. We engineered S. cerevisiae to produce vanillin, the compound that is responsible for vanilla flavor and aroma, from ferulic acid. Adaptive laboratory evolution of R.…